Mechanisms
Radiation inactivation puts the functional size of CETP at 70 000 daltons, consistent with a monomer (FEBS Lett 1985)
Original title: Cholesteryl ester transfer protein. Size of the functional unit determined by radiation inactivation
Radiation inactivation was applied to rabbit plasma from control and irradiated animals to determine the functional molecular weight of CETP, a technique that reveals the size of the functional unit needed to carry out the transfer reaction. The functional molecular weight was calculated at 70 000 plus or minus 3,000 for both control and irradiated rabbits, agreeing with the molecular weight obtained by the entirely different method of SDS-polyacrylamide gel electrophoresis on partially purified (110-fold) rabbit CETP. Isoelectric focusing of this CETP preparation gave a pI of 5.95. Together, the results indicate that the functional unit of CETP is the monomer rather than a larger oligomeric complex.
Original abstract
Radiation inactivation was used to determine the functional Mr of cholesteryl ester transfer protein (CETP) in rabbit plasma from control and irradiated animals. This technique reveals the size of the functional unit required to carry out the transfer function. The functional Mr was calculated to be 70 000 +/- 3000 (mean +/- SD) for both control and irradiated rabbits. This result is in accordance with the Mr obtained by a completely different method, namely SDS-polyacrylamide gel electrophoresis of a partially purified (110-fold) rabbit CETP. The pI of this CETP was found by isoelectric focusing to be equal to 5.95. The results suggest that the functional unit of this enzyme is the monomer.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 18 August 2026. Methods.