The class
A chimeric anti-CETP vaccine shrinks aortic plaques by over 40 percent in cholesterol-fed rabbits (Life Sci 2005)
Original title: Antibody against cholesteryl ester transfer protein (CETP) elicited by a recombinant chimeric enzyme vaccine attenuated atherosclerosis in a rabbit model
New Zealand White rabbits were vaccinated with a recombinant chimeric enzyme, rAnsB-TTP-CETPC, combining asparaginase, a tetanus toxin helper T-cell epitope, and a CETP B-cell epitope, in alum adjuvant, then fed a high cholesterol diet for 15 weeks to allow atherosclerotic lesions to form. After anti-CETP antibodies were successfully produced, rabbits showed an increased fraction of plasma cholesterol carried in HDL and a decreased fraction in LDL. Average aortic atherosclerotic plaque size in rAnsB-TTP-CETPC-immunized rabbits was 42.3% smaller than in ovalbumin-treated controls and 47.6% smaller than in rHSP65-treated negative controls, and coronary artery hyperplastic thickness was significantly lower (159 micrometers) than in rHSP65-immunized (187 micrometers) or ovalbumin-immunized (248 micrometers) rabbits. The authors conclude rAnsB-TTP-CETPC significantly attenuates atherosclerosis development and may be developed into an anti-atherosclerosis vaccine.
Original abstract
The recombinant chimeric enzyme of AnsB-TTP-CETPC, which comprised asparagianse, tetanus toxin helper T-cell epitope and CETP B-cell epitope, was used to vaccinate New Zealand white rabbits in alum adjuvant. After anti-CETP antibodies were successfully produced, rabbits were fed with a high cholesterol diet for fifteen weeks until atherosclerotic lesions formed in arteries. The results showed that after CETP was inhibited by anti-CETP antibodies the fraction of plasma cholesterol in HDL increased and the fraction of plasma cholesterol in LDL decreased in rAnsB-TTP-CETPC immunized rabbits. The average size of aorta atherosclerotic plaques in rabbits treated with rAnsB-TTP-CETPC was 42.3% less than in rabbits treated with OVA (neutral control), or 47.6% less than in rabbits treated with rHSP 65 (negative control). The average thickness of hyperplastic coronary artery in rAnsB-TTP-CETPC immunized rabbits was 159+/-12 microm, which was significantly lower than in rHSP 65 immunized rabbits (187+/-15 microm) or OVA immunized rabbits (248+/-18 microm). The data reported here demonstrated that rAnsB-TTP-CETPC could significantly attenuate the development of atherosclerosis in rabbits fed with high cholesterol diet, and might be developed to an anti-atherosclerosis vaccine in the future.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 19 August 2026. Methods.