Mechanisms
A wild valerian relative and beta-sitosterol inhibit CETP and lower lipids in triton-induced hyperlipidemic rats (Food Sci Nutr 2024)
Original title: Assessing lipid-lowering and plasma cholesteryl ester transfer protein activity of Centranthus longiflorus and β-Sitosterol following administration to triton WR1339- treated rats
Hyperlipidemia was induced in male Wistar rats by intraperitoneal Triton WR-1339, and animals across eight groups of six were pretreated by oral gavage with ethanol extract of Centranthus longiflorus, an aqueous suspension of beta-sitosterol, or anacetrapib before the triton injection, with blood biochemistry and liver histopathology assessed afterward. Both the Centranthus longiflorus extract and beta-sitosterol reduced total cholesterol similarly, with beta-sitosterol lowering LDL cholesterol more than the plant extract, and both normalized elevated ALT and AST liver enzymes, with the plant extract offering better protection of liver tissue histology. Both treatments also inhibited plasma CETP activity, with beta-sitosterol proving more effective than the Centranthus longiflorus extract, indicating their lipid-lowering effect is at least partly attributable to anti-CETP activity.
Original abstract
The aim of this study was to evaluate the lipid-lowering and plasma cholesteryl ester transfer protein(CETP) activity of Centranthus longiflorus(CL) and β-Sitosterol(βS) following intraperitoneal administration of Triton-WR 1339 (=Tyloxapol) (TWR) to male Wistar rats. Hyperlipidemia(HL) was developed by intraperitoneal injection of TWR. The animals were divided into main eight groups of six rats each. Rats were housed in separate cages and fed a standard diet for 7 days. After 7 days, ethanol extraction of CL plant, aqueous suspension of βS and anacetrapib was given to rats by oral gavage 1 h before the triton injection. Blood samples were collected and used for the biochemical parameters analysis. Histopathological studies were also performed on liver tissue. In hyperlipidemic rats(HR), CL extract and βS reduced total cholesterol similarly. βS lowered low-density lipoprotein(LDL-C) more than CL extract. Both CL extract and βS approximated impaired Alanine transaminase(ALT) and Aspartate transaminase(AST) levels in HR's to the level of control. CL extract provided better protection than βS against deterioration in liver tissue samples seen in hyperlipidemic rats. Finally, CL extract and βS inhibited CETP, at which point βS was more effective. These findings showed that CL extract and βS reduce plasma lipid concentration and may have a hypolipidemic effect due to their anti-CETP properties.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 18 August 2026. Methods.