Mechanisms
Doubling plasma CETP activity via diet does not speed cholesteryl ester clearance from hepatic reticuloendothelial cells in hamsters (Biochim Biophys Acta 1991)
Original title: Removal of cholesteryl ester from hepatic reticuloendothelial cells in vivo is not enhanced by plasma cholesteryl ester transfer protein
The putative role of cholesteryl ester transfer protein (CETP) in removing cholesteryl ester from hepatic reticuloendothelial cells in vivo was studied in hamsters, using retention of the nonhydrolysable cholesteryl ester analog [3H]cholesteryl linoleyl ether ([3H]CLE) after injecting [3H]CLE-labeled acetylated LDL, which targets nonparenchymal littoral cells. In chow-fed hamsters, plasma cholesteryl ester transfer activity (CETA) was 10.6 +/- 0.9 units, and 28-day hepatic [3H]CLE retention was 86% of the 4-hour value, versus about 55% in rats, in which CETA was undetectable. Supplementing the diet with 2% cholesterol and 15% margarine doubled hamster CETA, yet hepatic [3H]CLE retention at 28 days was unchanged; dietary fat supplementation also did not change retention in rats. These results do not support a role for CETP in vivo in removing cholesteryl ester from intact reticuloendothelial cells.
Original abstract
The putative role of cholesteryl ester transfer protein (CETP) in the removal of cholesteryl ester from hepatic reticuloendothelial cells in vivo was studied in hamsters. The parameter tested was retention of [3H]cholesteryl linoleyl ether ([3H]CLE), a nonhydrolysable analog of cholesteryl ester, in the liver after injection of [3H]CLE labeled acetylated LDL, which is targetted to nonparenchymatous littoral cells. In hamsters fed laboratory chow, plasma cholesteryl ester transfer activity (CETA) was 10.6 +/- 0.9 units and the retention of [3H]CLE in the liver 28 days after injection was 86% of the 4 h value. It was about 55% in rats fed the same diet, in which CETA was not detectable. When the diet was supplemented with 2% cholesterol and 15% margarine, CETA activity in hamsters increased 2-fold, yet no change in retention of [3H]CLE in liver was seen after 28 days. In rats, the retention of [3H]CLE in the liver was also not changed by the dietary fat supplementation. These results do not support the role of CETP in vivo in removal of cholesteryl ester from intact reticuloendothelial cells.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 19 August 2026. Methods.