Mechanisms
A fast two-site immunoassay measures CETP mass in 6 hours with under 9% assay variability (Clin Chem 1994)
Original title: Two-site enzyme immunoassay of cholesteryl ester transfer protein with monoclonal and oligoclonal antibodies
A sandwich-type enzyme immunoassay for CETP mass was developed using monoclonal antibody TP-4, recognizing a C-terminal epitope, for antigen capture and a peptide antibody against residues 290-306 for detection, revealed with an anti-rabbit-IgG peroxidase conjugate. Adding Triton X-100 to the incubation buffer increased antigen exposure, and standard-plasma and partially purified CETP calibration curves ran parallel. The assay delivered results within 6 hours, with mean intra- and interassay coefficients of variation of 3.6% and 8.4%, sensitivity at microgram concentrations, and a working range of 20-200 micrograms/L. In 40 normolipidemic subjects, mean plasma CETP was 1.1 plus or minus 0.4 mg/L, correlating strongly with CETP activity (r equals 0.91, n equals 42), with most plasma CETP found in the HDL fraction, establishing a rapid, precise tool for studying CETP in disease.
Original abstract
We developed a sandwich-type enzyme immunoassay to measure cholesteryl ester transfer protein (CETP) mass in human plasma. A specific monoclonal antibody (TP-4) that recognizes an epitope located in the C-terminal domain was used for antigen capture and an anti-CETP peptide antibody directed against the 290-306 residue was used for detection. Bound antibodies were revealed with an antibody-peroxidase conjugate specific for rabbit IgG. The presence of 10 mL/L Triton X-100 in the incubation buffer increased antigen exposure of CETP in plasma. The curves for CETP in standard plasma and partially purified CETP were parallel. This technique is rapid (results within 6 h), accurate, precise (mean intra- and interassay CVs 3.6% and 8.4%, respectively), and simple to perform. Assay sensitivity is at microgram concentrations, with a working range of 20-200 micrograms/L. In 40 normolipidemic healthy subjects, the mean CETP concentration in plasma was 1.1 +/- 0.4 mg/L. A strong correlation between CETP concentration and CETP activity (r = 0.91, n = 42) was observed. In plasma, the bulk of CETP was found in high-density lipoprotein fractions. Therefore, this assay may be a useful tool for investigations of CETP and its significance in relevant diseases.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 18 August 2026. Methods.