Genetics
A new simple sandwich immunoassay for CETP mass correctly detects genetic CETP deficiency in human serum (Clin Chim Acta 1998)
Original title: Enzyme immunoassay for cholesteryl ester transfer protein in human serum
This study developed a new simple sandwich-type enzyme immunoassay to measure CETP mass in human serum. Intra- and inter-assay coefficients of variation were 2.7-5.7% and 2.2-12.2% respectively, with no cross-reactivity against apoA-I, apoA-II, apoB, or apoC-III, and good correlation between CETP mass and CETP activity (n=46, r=0.88). Average CETP mass in normal serum was 1.8 microgram/mL in 41 males and 2.0 microgram/mL in 37 females. In patients with CETP gene mutations (intron 14A and D442G), the plasma CETP mass results from this assay correctly reflected genetic CETP deficiency. The authors conclude this assay provides a specific, reproducible, and clinically useful tool for measuring CETP mass in lipid metabolism research.
Original abstract
We developed a new simple sandwich-type enzyme immunoassay to measure cholesteryl ester transfer protein (CETP) mass in human serum. In assay validation, Intra- and Inter-assay coefficients of variation were 2.7 to 5.7% and 2.2 to 12.2%, respectively. There was no cross-reactivity with various lipoproteins (apo A-I, apo A-II, apo B, apo C-III). A good correlation between CETP mass and CETP activity (n = 46, correlation coefficient = 0.88) was observed. This assay provided a specific and reproducible method for measuring CETP mass in samples. The average value of CETP in the normal sera of 41 males was 1.8+/-0.6 microg/ml (mean+/-S.D.) and that of 37 females was 2.0+/-0.5 microg/ml. In the study of patients with the CETP gene mutation (Int 14A and D442G), our results on the value of plasma CETP mass reflected to genetic CETP deficiency. In conclusion, this assay for CETP mass in human serum may be a useful tool for clinical investigations involving lipid metabolism related to disease.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 19 August 2026. Methods.