Torcetrapib
A new LC-MS/MS assay quantifies torcetrapib in hamster and dog plasma down to 1 nanogram per mL (Biomed Chromatogr 2008)
Original title: Development and validation of a sensitive LC-MS/MS method with electrospray ionization using multiple ions for quantitation of torcetrapib in hamster and dog plasma
Researchers developed and validated a sensitive LC-MS/MS method for measuring torcetrapib in 100 microliters of hamster or dog plasma, using DRL-16126 as an internal standard on an API-4000 Q Trap instrument operated in multiple-reaction monitoring mode with electrospray ionization. Extraction with acetonitrile gave recoveries of 65.73% and 94.01% for torcetrapib and 79.68% and 90.70% for the internal standard in hamster and dog plasma respectively, with a 3.0-minute total chromatographic run time. The method was accurate and precise over a linearity range of 1.00 to 200 ng/mL with a correlation coefficient of at least 0.993, a lower limit of quantitation of 1.00 ng/mL, and torcetrapib remained stable across a battery of stability tests. The assay was successfully applied to preclinical pharmacokinetic studies in hamsters and dogs.
Original abstract
A highly sensitive and specific LC-MS/MS method has been developed and validated for the estimation of torcetrapib (TTB) with 100 microL hamster/dog plasma using DRL-16126 as an internal standard (IS). The API-4000 Q Trap LC-MS/MS was operated under multiple-reaction monitoring mode using the electrospray ionization technique. The assay procedure involved extraction of TTB and IS from plasma with acetonitrile, which yielded consistent recoveries of 65.73 and 94.01% for TTB and 79.68 and 90.70% for IS in hamster and dog plasma, respectively. The total chromatographic run time was 3.0 min and the elution of TTB and IS occurred at approximately 2.25 and 2.20 min, respectively. The resolution of peaks was achieved with 0.01 m ammonium acetate:acetonitrile (15:85, v/v) at a flow rate of 0.40 mL/min on an Inertsil ODS-3 column. The method was proved to be accurate and precise at linearity range of 1.00-200 ng/mL with a correlation coefficient (r) of > or = 0.993. The method was rugged with 1.00 ng/mL as the lower limit of quantitation. TTB was stable in the battery of stability studies. The application of the assay to preclinical pharmacokinetic studies confirmed the utility of the assay to derive hamster/dog pharmacokinetic parameters.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 18 August 2026. Methods.