Evacetrapib
A chiral HPLC method resolves the methyl ester of evacetrapib from its stereoisomers and five impurities (J Chromatogr A 2015)
Original title: High performance liquid chromatographic enantioseparation development and analytical method characterization of the carboxylate ester of evacetrapib using an immobilized chiral stationary phase with a non-conventional eluent system
Using multiple HPLC chromatographic modes and various chiral columns in an automated screening system, the authors identified a separation for the methyl ester of evacetrapib and its stereoisomers on an immobilized polysaccharide-based column, the Chiralpak IC. The bonded nature of this column enabled a diverse solvent range not compatible with standard coated chiral stationary phases, and the ternary eluent system ultimately identified achieved isomer resolutions not obtainable with established hexane/alcohol or polar organic chromatographic modes. The paper describes the systematic development process, first resolving the isomers and then incorporating five potential impurities, resulting in a robust separation system that resolves all compounds within a reasonable analysis time.
Original abstract
Using multiple HPLC chromatographic modes and various chiral columns in the context of an automated screening system, a potential separation was initially identified for the methyl ester of evacetrapib and its stereoisomers using an immobilized polysaccharide-based HPLC column. The bonded nature of this column, the Chiralpak(®) IC, allows for enhanced separation development with a diverse solvent range not amenable to standard coated chiral stationary phases. The ternary eluent system ultimately identified provided isomer resolutions not obtainable via the more established hexane/alcohol or polar organic chromatographic modes. A systematic separation development process is described, first for the resolution of the isomers, and later incorporating five potential impurities. A robust separation system was eventually developed that effectively resolves all compounds within a reasonable analysis time.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 18 August 2026. Methods.