HDL biology
A new sandwich ELISA measures plasma CETP concentration with under 10% assay variability (Clin Biochem 1999)
Original title: Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration
The authors developed a cost- and time-effective sandwich enzyme-linked immunosorbent assay for plasma CETP concentration, using one monoclonal anti-CETP antibody (TP20) for capture and a biotinylated monoclonal anti-CETP antibody (TP2) for detection, with results expressed as ng biotin-TP2 bound per microliter of plasma. Tested in 35 children with insulin-dependent diabetes mellitus, the assay showed an intra-assay coefficient of variation of 8.75% and an inter-assay coefficient of variation under 10%. Plasma CETP concentration ranged from 0.36 to 1.89 ng biotin-TP2 per microliter and was significantly correlated with CETP activity (r=0.51, P<0.01). The authors conclude that the sandwich ELISA has sufficient sensitivity for measuring plasma CETP concentration in humans.
Original abstract
Objectives: Cholesteryl ester transfer protein (CETP) mediates the transfer of HDL cholesterol to apoB-containing lipoproteins. Its mass and activity are increased in several pro-atherogenic conditions. The objective of this study is to develop a cost- and time-effective sandwich ELISA for plasma CETP concentration.
Design And Methods: Monoclonal anti-CETP, TP20, was used as the capture antibody, while the other biotinylated monoclonal anti-CETP, TP2, was used for detection. The results were expressed in an arbitrary unit, ng biotin-TP2 bound per microl plasma. Plasma CETP concentrations, activities and their relationship were assessed in 35 IDDM children.
Results: The assay had an intra-assay CV of 8.75% and an inter-assay CV under 10%. Plasma CETP concentration of these subjects ranged from 0.36-1.89 ng biotin-TP2/microL. CETP concentration was significantly correlated with CETP activity (r = 0.51, p < 0.01).
Conclusion: The sandwich ELISA we have developed carried sufficient sensitivity for assaying plasma CETP concentration in human.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 19 August 2026. Methods.