Genetics
CETP TaqIB B2 allele carriers have higher HDL cholesterol and antioxidant enzyme activity in both healthy and diabetic Argentinians (J Diabetes Investig 2015)
Original title: Association of cholesteryl ester transfer protein genotypes with paraoxonase-1 activity, lipid profile and oxidative stress in type 2 diabetes mellitus: A study in San Luis, Argentina
This study assessed associations of CETP TaqIB genotype (B1 and B2 alleles, rs708272) with lipoprotein profile, oxidative stress status, and paraoxonase-1 (PON-1) enzyme activity in 40 patients with type 2 diabetes mellitus and 30 healthy controls from San Luis, Argentina. Diabetic patients had significantly higher oxidative stress markers (TBARS and catalase activity, both p less than 0.0001) and significantly lower PON-1 activity and nitric oxide levels than controls (p=0.0002 and p=0.0008). CETP genotype distribution did not differ significantly between groups, but B2 allele carriers had higher HDL-cholesterol and PON-1 activity in both controls and diabetic patients. A significant positive correlation was found between PON-1 activity and HDL-cholesterol in B2 carriers, in both controls (r=0.83, P less than 0.0001) and diabetic patients (r=0.39, P=0.0003). The authors suggest lipoprotein and PON-1 alterations in diabetes may relate to higher CETP activity from insulin resistance.
Original abstract
Aims/Introduction: Diabetic dyslipidemia is common in type 2 diabetes. The TaqIB polymorphism in cholesteryl ester transfer protein (CETP; B1 and B2 alleles; rs708272) is associated with changes in enzyme activity and lipid concentrations. The aim of the present study was to assess associations of CETP genotypes with lipoprotein profile, oxidant/anti-oxidant status and the plasma activity of paraoxonase-1 (PON-1) in a population of diabetic patients living in San Luis, Argentina.
Materials And Methods: For oxidative stress status parameters, thiobarbituric acid-reactive substances (TBARS) and nitric oxide (NO) levels, and catalase and PON-1 activity were assessed in 40 patients with type 2 diabetes mellitus and 30 healthy participants. CETP polymorphism was analyzed by polymerase chain reaction-based methods.
Results: Type 2 diabetes mellitus had significantly higher concentrations of oxidative stress parameters: TBARS (P < 0.0001) and catalase activity (P < 0.0001). PON-1 activity and NO levels were significantly lower in diabetics (P = 0.0002 and P = 0.0008, respectively). The CETP genotypes distribution among study groups was not significantly different. The B2 carriers of the TaqIB CETP polymorphism are associated with higher high-density lipoprotein cholesterol levels and PON-1 activity in control and type 2 diabetes mellitus patients. Linear regression analysis showed that there was a significant and positive correlation between the changes of PON-1 activity and high-density lipoprotein cholesterol levels in non-B1B1 (B2 carriers) in controls (r = 0.83, P < 0.0001) and diabetic patients (r = 0.39, P = 0.0003).
Conclusions: The results of the current study show that type 2 diabetes mellitus is characterized by intense oxidative stress, and that the alterations observed in the lipoprotein profile and PON-1 activity might be related to the higher CETP activity in diabetic patients as a consequence of insulin resistance.
Summary written by cetpinhibition.org from the published abstract; figures as published. Page updated 19 August 2026. Methods.